PCR Enzymes and Kits
Standard PCR PCR Enzyme and Mastermix Catalog
ACTaq™ DNA Polymerase
ACTaq™ Blue DNA Polymerase
ACTaq™ Red DNA Polymerase
ACTaq™ DNA Polymerase P/N: E2100
Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with a molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75oC. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.
Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.
Concentration: 5 Units/μL
Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72oC.
Product Components: ACTaq™ DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2. (Buffer and MgCl2 concentrations are customizable upon request).
Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.
PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
Quality Control: ACTaq™ DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.
ACTaq™ Blue DNA Polymerase
ACTaq™ Red DNA Polymerase
P/N:E2100B (blue), E2100R (red)
Product Description: ACTaq™ DNA Polymerase originates from Thermus Aquaticus with molecular
weight of 94KDa. ACTaq™ Blue or Red differs from ACTaq™ DNA Polymerase in that it contains blue / red dyeadded to the enzyme used as tracking and loading dye as no loading buffer is required.
Storage Condition: -20oC. Stable for 12 months in constant freezer temperature.
Concentration: 1 Unit/μL
Unit Definition: One unit of the amount of enzyme that incorporates 10nmols of dNTPs into
acid-insoluble material in 30 minutes at 72oC.
Product Components: ACTaq™ Blue or Red DNA Polymerase, PCR reaction buffer, 10X 25mM MgCl2.
(Buffer and MgCl2 concentrations are customizable upon request)
Storage Buffer: 10mM Tris (pH 8.2), 50mM KCL, 0.5% BSA, 0.5% NP-40, 50% glycerol.
PCR Reaction Buffer: 10x PCR buffer with Mg2+. 15mM MgSO4, 100mM KCl, 80mM
(NH4)2SO4, 100mM Tris-HCl, pH 9.0, 0.5%NP-50.
Quality Control: ACTaq™ Blue or Red DNA Polymerase is highly purified. Free of contaminating
endonucleases, exonucleases, and nicking activities. The purity of the
enzyme is evaluated by SDS-PAGE at >95% purity.